Review



p p38mapk  (Bioss)


Bioz Verified Symbol Bioss is a verified supplier
Bioz Manufacturer Symbol Bioss manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Bioss p p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 40 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/P38+MAPK+(Thr180+%2B+Tyr182)+Antibody/pmc13083299-41-45-52
    Average 94 stars, based on 40 article reviews
    p p38mapk - by Bioz Stars, 2026-10
    94/100 stars

    Images

    1) Product Images from "Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway"

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    Journal: Journal of Traditional and Complementary Medicine

    doi: 10.1016/j.jtcme.2025.06.001

    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    Figure Legend Snippet: Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.

    Techniques Used: Transmission Assay, Electron Microscopy, Western Blot

    Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.
    Figure Legend Snippet: Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.

    Techniques Used: Transmission Assay, Electron Microscopy, Western Blot

    Schematic illustration summarizing the protective effects of OST against TRZ-induced cardiotoxicity by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway.
    Figure Legend Snippet: Schematic illustration summarizing the protective effects of OST against TRZ-induced cardiotoxicity by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway.

    Techniques Used:

    Related Articles

    Transmission Assay:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Electron Microscopy:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Western Blot:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Comparison:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Control:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Expressing:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Immunohistochemical staining:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Standard Deviation:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Infection:

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: 3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).3-methyladenine (3-MA) was purchased from Aladdin Company (Shanghai, China).. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).. The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).The anti-rabbit IgG (H + L) secondary antibody (1:10000 dilution, SA5-35571) was purchased from Thermo Fisher Scientific Company (Waltham, USA).

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway
    Article Snippet: (3-MA) was purchased from Aladdin Company (Shanghai, China). .. The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase- Jo urn al Pr e-p roo f 5 3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA). .. The anti-rabbit IgG (H+L) secondary antibody (1:10000 dilution, SA5-35571) w

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE -/- Mice.
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Jianpi Huayu Prescription Prevents Atherosclerosis by Improving Inflammation and Reshaping the Intestinal Microbiota in ApoE −/− Mice
    Article Snippet: with 5% skim milk for 18 min at room temperature. .. Incubate primary antibodies (GAPDH (CST, 1:4000), β-actin (Servicebio, batch number AC220110015)) overnight at 4°C; TLR4 (Wuhan Proteintech, 66350-1-lg, 1:2000), MyD88 (Wuhan Proteintech, 67969-1-lg, 1:5000), JNK (Wuhan Proteintech, 66210-1-lg, 1:10000), p-JNK (Bioss, bsm-52462R, 1:1000), AP-1 (Wuhan Proteintech, 66590-1-lg, 1:20000), P38MAPK (Wuhan Proteintech, 1:2000), p-P38MAPK (Bioss, bs-5476R, 1:500), ZO-1 (Abcam, ab276131, 1:1000), Occludin1 (Wuhan Proteintech, 66378-1-Ig, 1:5000), Claudin1 (Abcam, ab307692, 1:1000), JAM-1 (Abcam, ab270446, 1:1000). .. Wash the membrane 6 times at room temperature, 5 min ea

    Article Title: Bmp8a is an essential positive regulator of antiviral immunity in zebrafish
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).

    Article Title: Bmp8a Is a Novel Player in Regulation of Antiviral Immunity
    Article Snippet: from Cell Signaling Technology: TBK1 (1:1000, #3504T), p-TBK1(Ser172) (1:1000, #5483T). .. Antibodies from Bioss: IRF3 (1:1000, #bs-2993R), p-IRF3 (Ser386) (1:1000, #bsm-52170R), p38MAPK (1:1000, #bs-0637R), p-p38MAPK (Thr180 + Tyr182) (1:1000, #bs-2210R), Actin (1:2000, #bs-0061R). .. Antibodies from Beyotime: HA tag (1:1000, #AH158) and Flag tag (1:1000, #AF519).



    Similar Products

    p p38mapk  (Bioss)
    94
    Bioss p p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk, supplied by Bioss, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/P38+MAPK+(Thr180+%2B+Tyr182)+Antibody/pmc13083299-41-45-52
    Average 94 stars, based on 1 article reviews
    p p38mapk - by Bioz Stars, 2026-10
    94/100 stars
    		  Buy from Supplier
    p p38mapk  (Proteintech)
    96
    Proteintech p p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/p38+MAPK+Antibody/pm41921866-105-27-29
    Average 96 stars, based on 1 article reviews
    p p38mapk - by Bioz Stars, 2026-10
    96/100 stars
    		  Buy from Supplier
    p p38mapk  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc p p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/pm41811890-78-23-46
    Average 86 stars, based on 1 article reviews
    p p38mapk - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    p p38mapk thr180 tyr182  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc p p38mapk thr180 tyr182
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk Thr180 Tyr182, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/pm41645235-268-18-7
    Average 86 stars, based on 1 article reviews
    p p38mapk thr180 tyr182 - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    antibodies p p38mapk  (ABclonal Biotechnology)
    98
    ABclonal Biotechnology antibodies p p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    Antibodies P P38mapk, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/%CE%B2-Tubulin+Rabbit+mAb/pm41654165-241-55-56
    Average 98 stars, based on 1 article reviews
    antibodies p p38mapk - by Bioz Stars, 2026-10
    98/100 stars
    		  Buy from Supplier
    p-p38mapk  (ABclonal Biotechnology)
    95
    ABclonal Biotechnology p-p38mapk
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    P P38mapk, supplied by ABclonal Biotechnology, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/Phospho-p38+MAPK-T180%2FY182+Rabbit+pAb/pm41654165-241-39-41
    Average 95 stars, based on 1 article reviews
    p-p38mapk - by Bioz Stars, 2026-10
    95/100 stars
    		  Buy from Supplier
    sprague dawley adult rats acute pe inhibited tnf α induced p p38mapk expression  (Dawley Inc)
    86
    Dawley Inc sprague dawley adult rats acute pe inhibited tnf α induced p p38mapk expression
    Effects of OST on cardiac autophagy and the <t>p38MAPK/mTOR</t> signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.
    Sprague Dawley Adult Rats Acute Pe Inhibited Tnf α Induced P P38mapk Expression, supplied by Dawley Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/adult+dawley+male+rats+sprague/pmc12872061-142-28-28
    Average 86 stars, based on 1 article reviews
    sprague dawley adult rats acute pe inhibited tnf α induced p p38mapk expression - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    p thr180 tyr182 p38mapk  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc p thr180 tyr182 p38mapk
    ( a ) Confirmation of a physical interaction between TMA and IRAK4 using TMA at concentrations ranging from 0.1 nM to 100 µM, resulting in a dissociation constant ( K d ) of 14 nM. ( b – e ) Physical interaction test between TMA and the four hits identified using a kinome screen, using TMA concentrations ranging from 0.1 nM to 100 µM for ( b ) ANKK1, ( c ) HIPK2, ( d ) JAK2 and ( e ) NDR1. For (b-e) N = 2 biological repeats. IRAK4 kinase activity is not significantly inhibited by choline ( f ), TMAO ( g ) or DMB ( h ), while TMA does not have any inhibitory effect on IRAK-1 kinase activity (average of N = 2 biological repeats) ( i ). ( j , k ) Phosphorylation for pThr183/Tyr185 SAPK/JNK/SAPK/JNK ( j ) and <t>pThr180/Tyr182</t> <t>p38MAPK/p38MAPK</t> ( k ) densitometric ratios, represented by each dot. Data are means ± s.e.m. One-way ANOVA followed by Tukey’s post hoc tests (superscript letters for factor levels P < 0.05) on log-transformed data. Source data are provided.
    P Thr180 Tyr182 P38mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/pmc12727497-215-27-37
    Average 86 stars, based on 1 article reviews
    p thr180 tyr182 p38mapk - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier
    phosphor p38mapk p p38mapk antibodies  (Cell Signaling Technology Inc)
    86
    Cell Signaling Technology Inc phosphor p38mapk p p38mapk antibodies
    ( a ) Confirmation of a physical interaction between TMA and IRAK4 using TMA at concentrations ranging from 0.1 nM to 100 µM, resulting in a dissociation constant ( K d ) of 14 nM. ( b – e ) Physical interaction test between TMA and the four hits identified using a kinome screen, using TMA concentrations ranging from 0.1 nM to 100 µM for ( b ) ANKK1, ( c ) HIPK2, ( d ) JAK2 and ( e ) NDR1. For (b-e) N = 2 biological repeats. IRAK4 kinase activity is not significantly inhibited by choline ( f ), TMAO ( g ) or DMB ( h ), while TMA does not have any inhibitory effect on IRAK-1 kinase activity (average of N = 2 biological repeats) ( i ). ( j , k ) Phosphorylation for pThr183/Tyr185 SAPK/JNK/SAPK/JNK ( j ) and <t>pThr180/Tyr182</t> <t>p38MAPK/p38MAPK</t> ( k ) densitometric ratios, represented by each dot. Data are means ± s.e.m. One-way ANOVA followed by Tukey’s post hoc tests (superscript letters for factor levels P < 0.05) on log-transformed data. Source data are provided.
    Phosphor P38mapk P P38mapk Antibodies, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+p38mapk/pm41218475-54-3-9
    Average 86 stars, based on 1 article reviews
    phosphor p38mapk p p38mapk antibodies - by Bioz Stars, 2026-10
    86/100 stars
    		  Buy from Supplier

    Image Search Results


    Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    doi: 10.1016/j.jtcme.2025.06.001

    Figure Lengend Snippet: Effects of OST on cardiac autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-p38MAPK, p38MAPK, p-mTOR and mTOR. (H) Quantitative analysis of p-p38MAPK/p38MAPK and p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group.

    Article Snippet: The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot

    Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    doi: 10.1016/j.jtcme.2025.06.001

    Figure Lengend Snippet: Effects of OST on autophagy and the p38MAPK/mTOR signaling pathway in TRZ-treated mice after SB203580 administration. (A–B) Representative images of transmission electron microscopy images, yellow arrow, mitochondria; red arrow, autophagosome. (C) Representative western blotting bands for LC3I, LC3II, Beclin-1, p62. (D–F) Quantitative analysis of LC3II/I, Beclin-1, p62 (normalized to GAPDH). (G) Representative western blotting bands for p-mTOR and mTOR. (H) Quantitative analysis of p-mTOR/mTOR. Data are expressed as means ± SEM ( n = 3). # P < 0.05, ## P < 0.01 vs the CON group; ∗ P < 0.05, ∗∗ P < 0.01 vs the TRZ group; ▲ P < 0.05, ▲▲ P < 0.01 vs the TRZ + OST H group.

    Article Snippet: The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).

    Techniques: Transmission Assay, Electron Microscopy, Western Blot

    Schematic illustration summarizing the protective effects of OST against TRZ-induced cardiotoxicity by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway.

    Journal: Journal of Traditional and Complementary Medicine

    Article Title: Osthole attenuates trastuzumab-induced cardiotoxicity in mice by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway

    doi: 10.1016/j.jtcme.2025.06.001

    Figure Lengend Snippet: Schematic illustration summarizing the protective effects of OST against TRZ-induced cardiotoxicity by enhancing autophagy via regulating the p38MAPK/mTOR signaling pathway.

    Article Snippet: The first antibodies to Bax (1:1000 dilution, A0207), Bcl-2 (1:800 dilution, A0208), Caspase-3 (1:800 dilution, A2156), LC3 (1:500 dilution, A19665), Beclin-1 (1:1000 dilution, A7353), p62 (1:1000 dilution, A19700) and GAPDH (1:800 dilution, A19056) were purchased from ABclonal Company (Wuhan, China), p38MAPK (1:1000 dilution, bs-0637R) and p-p38MAPK (1:1000 dilution, bs-0636R) were purchased from Bioss Company (Beijing, China), mTOR (1:1000 dilution, #2983) and p-mTOR (1:1000 dilution, #2971) were purchased from CST Company (Danvers, MA, USA).

    Techniques:

    ( a ) Confirmation of a physical interaction between TMA and IRAK4 using TMA at concentrations ranging from 0.1 nM to 100 µM, resulting in a dissociation constant ( K d ) of 14 nM. ( b – e ) Physical interaction test between TMA and the four hits identified using a kinome screen, using TMA concentrations ranging from 0.1 nM to 100 µM for ( b ) ANKK1, ( c ) HIPK2, ( d ) JAK2 and ( e ) NDR1. For (b-e) N = 2 biological repeats. IRAK4 kinase activity is not significantly inhibited by choline ( f ), TMAO ( g ) or DMB ( h ), while TMA does not have any inhibitory effect on IRAK-1 kinase activity (average of N = 2 biological repeats) ( i ). ( j , k ) Phosphorylation for pThr183/Tyr185 SAPK/JNK/SAPK/JNK ( j ) and pThr180/Tyr182 p38MAPK/p38MAPK ( k ) densitometric ratios, represented by each dot. Data are means ± s.e.m. One-way ANOVA followed by Tukey’s post hoc tests (superscript letters for factor levels P < 0.05) on log-transformed data. Source data are provided.

    Journal: Nature Metabolism

    Article Title: Inhibition of IRAK4 by microbial trimethylamine blunts metabolic inflammation and ameliorates glycemic control

    doi: 10.1038/s42255-025-01413-8

    Figure Lengend Snippet: ( a ) Confirmation of a physical interaction between TMA and IRAK4 using TMA at concentrations ranging from 0.1 nM to 100 µM, resulting in a dissociation constant ( K d ) of 14 nM. ( b – e ) Physical interaction test between TMA and the four hits identified using a kinome screen, using TMA concentrations ranging from 0.1 nM to 100 µM for ( b ) ANKK1, ( c ) HIPK2, ( d ) JAK2 and ( e ) NDR1. For (b-e) N = 2 biological repeats. IRAK4 kinase activity is not significantly inhibited by choline ( f ), TMAO ( g ) or DMB ( h ), while TMA does not have any inhibitory effect on IRAK-1 kinase activity (average of N = 2 biological repeats) ( i ). ( j , k ) Phosphorylation for pThr183/Tyr185 SAPK/JNK/SAPK/JNK ( j ) and pThr180/Tyr182 p38MAPK/p38MAPK ( k ) densitometric ratios, represented by each dot. Data are means ± s.e.m. One-way ANOVA followed by Tukey’s post hoc tests (superscript letters for factor levels P < 0.05) on log-transformed data. Source data are provided.

    Article Snippet: Membranes were immunoblotted with antibodies against the following proteins: p Thr345/Ser346 IRAK4 (11927), IRAK4 (4363), p Ser176/180 IKKαβ (2694), IKKβ (8943), p Thr183/Tyr185 SAPK/JNK (4668), SAPK/JNK (9258), p Thr180/Tyr182 p38MAPK (9215) and p38MAPK (9212), all purchased from Cell Signaling Technology, and β-actin (sc-47778, Santa Cruz Biotechnology).

    Techniques: Activity Assay, Phospho-proteomics, Transformation Assay